FastQCFastQC Report
Wed 8 May 2024
EGAF00006128401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352874
Total Bases49.1 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26660.7555104654919319No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG5780.16379784285609028No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4420.12525717394877492No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4180.11845587943571927No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT3910.11080442310853165No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACGGTGGACACG3680.10428651586685332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)