FastQCFastQC Report
Wed 8 May 2024
EGAF00006128404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314366
Total Bases45.4 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT111403.5436402155449382TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9980.31746435683248186No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA8120.2582976530540835No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA5750.18290782082031773No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT4900.15586927339470552TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGGAT3630.11547050253526145TruSeq Adapter, Index 9 (97% over 36bp)
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3340.10624558635475848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)