FastQCFastQC Report
Wed 8 May 2024
EGAF00006128407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341934
Total Bases47.5 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23070.6746916071522575No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACATCAGCCCCTCTA4530.13248170699608697No Hit
GCATTTCCTTGCTTGCTCACTTCAGTCCCTCTAGCCTTCTTTCTGTCCCT4270.1248779004135301No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4180.1222458135195681No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA4150.12136845122158078No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3840.11230237414237836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)