FastQCFastQC Report
Wed 8 May 2024
EGAF00006128408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365060
Total Bases53.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT92112.523146879964937TruSeq Adapter, Index 11 (97% over 38bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA11530.31583849230263517No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT8800.24105626472360708No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6920.18955788089629103No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4370.11970634964115487No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT4150.11367994302306471TruSeq Adapter, Index 11 (97% over 38bp)
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3880.10628389853722675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)