FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365060
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT92092.522599024817838TruSeq Adapter, Index 11 (97% over 38bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA11430.3130992165671396No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT8750.23968662685585929No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6920.18955788089629103No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4350.11915849449405577No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT4150.11367994302306471TruSeq Adapter, Index 11 (97% over 38bp)
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3850.10546211581657809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)