FastQCFastQC Report
Wed 8 May 2024
EGAF00006128410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340538
Total Bases47.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT102733.016697108692716TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23780.6983067968919768No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG5770.1694377719960768No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4610.1353740258062243No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT4310.12656443627436584TruSeq Adapter, Index 9 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA4050.11892945868008857No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3970.11658023480492632No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA3930.1154056228673452No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTT3740.10982621616383488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)