FastQCFastQC Report
Wed 8 May 2024
EGAF00006128412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263911
Total Bases36.1 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT186717.07473352759074TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22240.842708337280371No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA5470.20726684374656607No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT4570.17316443801129927No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3860.14626142904236655No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3270.12390540750480275No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3130.11860058883487236No Hit
GCGTGACAGCCACTGTAGGACTCTGATCTCAGGGGGACAGGCTGACACAG3070.1163270951191879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)