FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263911
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT186647.072081118255776TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22240.842708337280371No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA5380.20385660317303939No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT4450.16861745057993036No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3800.1439879353266821No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3220.12201082940839904No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3090.11708492635774939No Hit
GCGTGACAGCCACTGTAGGACTCTGATCTCAGGGGGACAGGCTGACACAG3040.11519034826134567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)