FastQCFastQC Report
Wed 8 May 2024
EGAF00006128413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263911
Total Bases36.1 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22230.8423294216610903No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA5320.20158310945735497No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT4180.15838672885935032No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3440.13034697303257536No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3140.1189795044541531No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT2850.10799095149501158No Hit
GCGTGACAGCCACTGTAGGACTCTGATCTCAGGGGGACAGGCTGACACAG2670.1011704703479582No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)