FastQCFastQC Report
Wed 8 May 2024
EGAF00006128415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356299
Total Bases49.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22790.6396313208849871No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG6620.18579900589111953No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5570.1563293750473619No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4910.13780560708842854No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA4210.11815918652592346No Hit
GGCCTGGAGAGGCTCTGCGACCCGCTTAGGACCACAGAACTCGGTACTAG3770.10581000788663456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)