FastQCFastQC Report
Wed 8 May 2024
EGAF00006128417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326145
Total Bases44.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24770.7594781462233056No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC5600.17170277024023056No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4980.15269282067791934No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA4080.12509773260359655No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA3890.1192721028990173No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA3840.11773904245044382No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3700.11344647319443805No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA3550.1088472918487176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)