FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272579
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT157775.78804676809292TruSeq Adapter, Index 18 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20260.7432707582022093No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT6960.25533881920470763TruSeq Adapter, Index 18 (97% over 39bp)
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3950.1449121172210625No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA3520.12913687408054178No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3420.12546821288507184No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA3120.11446222929866202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)