FastQCFastQC Report
Wed 8 May 2024
EGAF00006128424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385594
Total Bases55.5 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT111542.892679865350602TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14900.3864168010913033No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA9360.24274236632312743No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG7280.18879961825132133No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT7010.18179743460738496No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA6380.16545900610486677No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA5920.1535293598966789No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT5770.1496392578722698No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTACTT4210.10918219681841522No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT4190.108663516548494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)