FastQCFastQC Report
Wed 8 May 2024
EGAF00006128429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327251
Total Bases44.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29670.9066435243895358No Hit
GCATTTCCTTGCTTGCTCACTTCAGTCCCTCTAGCCTTCTTTCTGTCCCT4420.13506452233912195No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACATCAGCCCCTCTA4340.13261991559995234No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4330.1323143397575561No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4100.12528609538244345No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3680.11245191000180291No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3500.10695154483867124No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)