FastQCFastQC Report
Wed 8 May 2024
EGAF00006128432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410556
Total Bases58.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT121702.964272839758766TruSeq Adapter, Index 11 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18330.4464677169496975No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG7440.18121766579954987No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT7270.17707693956488274No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA6080.1480918559222128No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA5980.14565613460770271No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5940.1446818460818987No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA5680.13834897066417248No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT5430.1322596673778973TruSeq Adapter, Index 11 (97% over 38bp)
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA5160.12568321982872008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)