FastQCFastQC Report
Wed 8 May 2024
EGAF00006128433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410556
Total Bases58.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18710.45572345794483576No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT5960.1451689903448007No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG5550.13518253295530938No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5170.1259267919601711No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA5100.12422178704001403No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4370.10644102144409046No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA4360.10619744931263944No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4120.10035171815781524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)