FastQCFastQC Report
Wed 8 May 2024
EGAF00006128434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311864
Total Bases43.3 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT191936.154285201241567TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20530.6582997716953544No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA3960.12697842649359978No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3950.12665777390144423No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3800.12184798501911089No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA3560.11415232280737758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)