FastQCFastQC Report
Wed 8 May 2024
EGAF00006128437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311001
Total Bases44 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15130.4864936125607313No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA7020.22572274687219654No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC6630.21318259426818562No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA6040.19421159417493836No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA5710.18360069581769833No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4550.1463017803801274No Hit
CTGAAGTAGATGAACAGCCCTGTCCCAAGGAAGAGCAGGCCCAGCACAAA4070.1308677464059601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)