FastQCFastQC Report
Wed 8 May 2024
EGAF00006128442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263678
Total Bases35.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT211878.035179271687436TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23560.8935140588141597No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT8830.334878146830604TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)