FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339998
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT119503.514726557215042TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17400.5117677162806841No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA6290.18500108824169556No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5930.1744127906634745No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4430.13029488408755346No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA3910.1150006764745675No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3400.10000058823875435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)