FastQCFastQC Report
Wed 8 May 2024
EGAF00006128445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339998
Total Bases48.4 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17270.5079441643774375No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5360.1576479861646245No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA5030.14794204671792185No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4090.12029482526367802No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA3410.10029470761592715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)