FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326775
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT147944.527274118277102TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18170.556040088746079No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC10570.3234641572947747No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4770.14597199908193711No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA4760.1456659781195012No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4510.13801545405860302No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4290.13128299288501263No Hit
GGCCTGGAGAGGCTCTGCGACCCGCTTAGGACCACAGAACTCGGTACTAG4040.12363246882411444No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA3790.11598194476321627No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA3700.11322775610129293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)