FastQCFastQC Report
Wed 8 May 2024
EGAF00006128447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326775
Total Bases45.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18390.5627725499196695No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC10080.30846913013541427No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4960.15178639736821972No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4530.13862749598347487No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA4300.13158901384744853No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4270.13067095096014075No Hit
GGCCTGGAGAGGCTCTGCGACCCGCTTAGGACCACAGAACTCGGTACTAG3990.12210236401193483No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA3710.11353377706372887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)