FastQCFastQC Report
Wed 8 May 2024
EGAF00006128449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316712
Total Bases45.3 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13660.4313066760968956No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA6120.193235494708126No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5250.16576574300942182No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4420.1395589684003132No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4330.13671726994872313No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3490.11019475106721566No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC3400.10735305261562555No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC3390.10703730834322667No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3280.10356412134683876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)