FastQCFastQC Report
Wed 8 May 2024
EGAF00006128450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336756
Total Bases47.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT127623.7896874888643413TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18120.5380750454334889No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA6220.1847034648231954No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT5850.17371628122438798No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT5330.15827483400444237No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA4660.13837912316335862No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA4300.12768889047262705No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTT4130.12264072503533716No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA4000.11878036323035077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)