FastQCFastQC Report
Wed 8 May 2024
EGAF00006128452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235329
Total Bases32.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT141956.0319807588525TruSeq Adapter, Index 18 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15730.6684259058594564No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT6570.2791836110296648TruSeq Adapter, Index 18 (97% over 39bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5380.2286161076620391No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4040.171674549248074No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC3430.14575339205962715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)