FastQCFastQC Report
Wed 8 May 2024
EGAF00006128454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352876
Total Bases48.8 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT139853.963148528094855TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27190.7705256237318492No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC6540.1853342250535599No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT4970.14084267561409672No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4130.11703827973565785No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4030.11420442308346275No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA3900.11052040943560912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)