FastQCFastQC Report
Wed 8 May 2024
EGAF00006128458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232445
Total Bases32.5 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT86573.7243218825958833TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11700.5033448772827981No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT5310.2284411366129622No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3530.15186388177848523No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC2920.12562111467228806No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG2850.12260964959452773No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA2660.11443567295489257No Hit
GGCCTGGAGAGGCTCTGCGACCCGCTTAGGACCACAGAACTCGGTACTAG2440.10497106842478866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)