FastQCFastQC Report
Wed 8 May 2024
EGAF00006128460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293814
Total Bases42.6 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT126824.316336185477888TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8040.2736425085258021No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT5310.18072658212338416TruSeq Adapter, Index 9 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA4300.14635109286827722No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA4220.14362828183816972No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3930.1337580918540301No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACGTCAGCCCCTCTA3130.10652998155295527No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA2980.10142471087150375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)