FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273737
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT146105.337239759331037TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27851.0173999130552318No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3540.12932120977434547No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3510.12822526731863065No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTT3110.11361270124243343No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA3040.11105550217909892No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA2980.10886361726766933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)