FastQCFastQC Report
Wed 8 May 2024
EGAF00006128464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312357
Total Bases43.9 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATCTCGTAT218486.994560710981346TruSeq Adapter, Index 7 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15880.5083926404722802No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATAGTTCAGTCCCTCTA5260.16839705849396686No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTCTCCATATCGTAT5220.167116472497815TruSeq Adapter, Index 7 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA4660.14918826855168926No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4070.13029962510844964No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT3220.10308717269022305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)