FastQCFastQC Report
Wed 8 May 2024
EGAF00006128466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332378
Total Bases47.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT165774.9873938708338095TruSeq Adapter, Index 2 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10830.3258338397848233No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5870.176606153235172No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA5030.15133372244853752No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4240.1275656030182503No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA3930.1182388726088971No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3750.11282335172604686No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC3350.10078886087526852No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)