FastQCFastQC Report
Wed 8 May 2024
EGAF00006128467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332378
Total Bases47.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10650.3204183189019731No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5430.16336821329931583No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA4440.13358284844363946No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4270.12846818983205868No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4150.12485784257682518No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA3960.11914145942270547No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT3330.1001871363327296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)