FastQCFastQC Report
Wed 8 May 2024
EGAF00006128468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313353
Total Bases46 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGCGACATCTCGTAT106403.3955315570618443TruSeq Adapter, Index 2 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA12210.3896563939071909No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA9510.3034915893576893No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6540.20871030435323742No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG5720.182541734082648No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4170.13307675369311928No Hit
CCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTT3860.12318375761521351No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACGGTGGACACG3250.10371689436514092No Hit
GTCCAAGTAGAGACAGTAAGTAGTTACAGGCACCCACCACACTGTGTTGT3140.10020647640201305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)