FastQCFastQC Report
Wed 8 May 2024
EGAF00006128472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332718
Total Bases48 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT131153.941776519454914TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9980.29995371455707237No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACATCAGCCCCTCTA6440.19355730678833125No Hit
CGGATCCTCCTCCAGCTCCTGCTTGGAGGTCTCCAGAATAGGCTGGAGGC4730.1421624318491936No Hit
AGACATGCACACACCAGAGAAGATTCCAATTTAATGTCCTCCCTTTATTC4210.1265335809905085No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3970.11932026520957689No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3940.11841860073696044No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3500.10519418847191916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)