FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332718
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT131083.939672635685475TruSeq Adapter, Index 8 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9980.29995371455707237No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACATCAGCCCCTCTA6350.1908523133704819No Hit
CGGATCCTCCTCCAGCTCCTGCTTGGAGGTCTCCAGAATAGGCTGGAGGC4700.14126076737657717No Hit
AGACATGCACACACCAGAGAAGATTCCAATTTAATGTCCTCCCTTTATTC4150.12473025204527557No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3920.11781749108854946No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3880.11661527179172752No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3470.10429252399930271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)