FastQCFastQC Report
Wed 8 May 2024
EGAF00006128473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences332718
Total Bases48 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10270.30866980445903136No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACATCAGCCCCTCTA6540.19656285503038606No Hit
CGGATCCTCCTCCAGCTCCTGCTTGGAGGTCTCCAGAATAGGCTGGAGGC4440.1334463419472346No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4050.12172470380322074No Hit
AGACATGCACACACCAGAGAAGATTCCAATTTAATGTCCTCCCTTTATTC3810.11451138802228915No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3720.11180639460443978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)