FastQCFastQC Report
Wed 8 May 2024
EGAF00006128474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252003
Total Bases36.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT118204.6904203521386645TruSeq Adapter, Index 18 (97% over 39bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA13330.5289619568021016No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8630.3424562406003103No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT6240.24761609980833563TruSeq Adapter, Index 18 (97% over 39bp)
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4410.17499791669146797No Hit
AGACATGCACACACCAGAGAAGATTCCAATTGAGTGTCCTCCCTTTATTC3910.15515688305297953No Hit
GTCCAAGTAGAGACAGTAAGTAGTTACAGGCACCCACCACACTGTGTTGT2570.10198291290183052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)