FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434760
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT132283.0425982151071858TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22910.5269574017848928No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA10820.2488729413929524No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT8360.19228999907995215No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA7270.16721869537215933No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT6090.14007728401876898TruSeq Adapter, Index 9 (97% over 36bp)
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA4500.10350538227987854No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4400.10120526267365904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)