FastQCFastQC Report
Wed 8 May 2024
EGAF00006128484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286956
Total Bases41.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT137564.793766291696288TruSeq Adapter, Index 11 (97% over 38bp)
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA10040.34987942402319516No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7860.2739095889265253No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT5930.20665189088222585TruSeq Adapter, Index 11 (97% over 38bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5850.2038640070254673No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3240.11290929619872037No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT3190.11116686878824629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)