FastQCFastQC Report
Wed 8 May 2024
EGAF00006128486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274868
Total Bases38.7 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT86463.1455098447254684TruSeq Adapter, Index 11 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15410.5606327400788742No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT4000.14552439716518475TruSeq Adapter, Index 11 (97% over 38bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA3980.14479677517935882No Hit
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG3880.14115866525022921No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCTTTCTA3710.13497387837070884No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3540.1287890914911885No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTACTT2890.10514137695184597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)