FastQCFastQC Report
Wed 8 May 2024
EGAF00006128490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317626
Total Bases44.5 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT82062.5835416496130668TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21050.6627291216713997No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA11460.3608016975940257No Hit
TGTCAAGTGGGAAATGAATGCTCTTGCAAGGCTCAAACTTGTGAACACAT5620.17693765623720978No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3790.11932272546957742No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA3730.1174337113460485No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT3500.1101924905391876TruSeq Adapter, Index 9 (97% over 36bp)
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTTCTTCAAGTCACAAAGGGA3330.10484028385585563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)