FastQCFastQC Report
Wed 8 May 2024
EGAF00006128492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268782
Total Bases37.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT117984.389430839862789TruSeq Adapter, Index 9 (97% over 36bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18050.6715479459189976No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT6460.24034347538153597No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA5510.20499884664895712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT4950.1841641181329107TruSeq Adapter, Index 9 (97% over 36bp)
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC4090.15216792791183933No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA3330.12389222492577628No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3100.11533510428525719No Hit
AGACATGCACACACCAGAGAAGATTCCAATTGAGTGTCCTCCCTTTATTC2710.10082520406872483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)