FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295986
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT176705.9698769536397TruSeq Adapter, Index 11 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27320.9230166291648929No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT5960.20136087517652865TruSeq Adapter, Index 11 (97% over 38bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTGCTATACTTCAGTCCCTCTA3700.12500591244180467No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA3340.11284317501503449No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT3190.10777536775388025No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT3180.10743751393646997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)