FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296380
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATCTCGTAT149255.0357648964167625TruSeq Adapter, Index 11 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21130.7129360955530063No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTATGAATATCGTAT5280.17814967271745732TruSeq Adapter, Index 11 (97% over 38bp)
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT4830.16296646197449222No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4400.1484580605978811No Hit
GAACCGGTAGCAACCAGGGCCTGAGAAAGTCCTCTCTTGTGGAAGAATAA3820.12888858897361496No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA3630.12247789999325191No Hit
GCAGGTGCCTTTGCAGAAACAAAGTCAGGGTACTTCAAGTCACAAAGGGA3100.10459545178487077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)