FastQCFastQC Report
Wed 8 May 2024
EGAF00006128502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257762
Total Bases35.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATCTCGTAT99093.844243914929276TruSeq Adapter, Index 18 (97% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23980.9303155624180446No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA5150.1997967116952848No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC4980.19320148043544044No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCTAATATCGTAT4940.19164966131547706TruSeq Adapter, Index 18 (97% over 39bp)
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC4300.16682055539606302No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4010.15556986677632856No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT3190.12375757481707932No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA3010.11677438877724412No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT2760.10707551927747302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)