FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257762
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24620.9551446683374586No Hit
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACGTCAGCCCTTCTA4070.15789759545627363No Hit
GGCAGACAGTGTGACAAAGAGGCTTGGTGTAGGAGAAGAGGGATCAGGAC3970.15401804765636518No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTC3800.1474228163965208No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA3370.13074076085691452No Hit
TGTCAAGTGGGAAATGAATGCTCTTACAAGGCTCAAACTTGTGAACACAT2870.1113430218573723No Hit
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACA2820.1094032479574181No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT2750.10668756449748217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)