FastQCFastQC Report
Wed 8 May 2024
EGAF00006128506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252474
Total Bases37.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT103544.101016342276829TruSeq Adapter, Index 8 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA6140.24319335852404605No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA5640.22338933910026382No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4850.19209898841068784No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3190.12634964392373077No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTT3020.1196162773196448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)