FastQCFastQC Report
Sat 6 Aug 2022
EGAF00006128506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252474
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTACGACATCTCGTAT103494.09903594033445TruSeq Adapter, Index 8 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCCTTGCTTTCTATACCTCAGCCCTTCTA6120.24240119774709476No Hit
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA5630.22299325871178813No Hit
CTCATCTTCTGCCTCTCCATTTCCTTACTTTCTATACATCAGCCCCTCTA4830.19130682763373655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3190.12634964392373077No Hit
CCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTT3020.1196162773196448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)