FastQCFastQC Report
Wed 8 May 2024
EGAF00006128508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006128508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329130
Total Bases48.2 Mbp
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGTAT128033.889952298483882TruSeq Adapter, Index 9 (97% over 36bp)
CTCATCTTCTGCCTCTCCATTTCGTTGCTTTCTATACTTCAGCCCCTCTA16740.5086136177194421No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7200.21875854525567404No Hit
CCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTT6060.18412177559019233No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATATCGTAT5770.17531066751739433TruSeq Adapter, Index 9 (97% over 36bp)
GGCAGACAGTGTGACAAAGAGGCTGGTGTAGGAGAAGAGGGATCAGGACG4750.14431987360617385No Hit
TGGATACTCACGACGCGGACCCAGTTCTCACTCCCATTGGGTGTCGGGTT4500.13672409078479628No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAGCGAATCTCGGAT3750.11393674232066356TruSeq Adapter, Index 9 (97% over 36bp)
CTGGGGAGGAAACACAGGTCAGCATGGGAACAGGGGTCACAGTGGACACG3500.10634095949928601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)